384 Awareness Technology ChroMate BioTek Synergy Mx. Molecular Devices gmbH Tel. Listing: #4772697. ValitaTiter 96-well (Pack of 20). LVF Monochromator™ : Excitation and Emission. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). #12-639) White 96- and 384-well microplates (Greiner cat. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. 0200-6262. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. Application Area: Spectrophotometer for molecular biology. Use black microplates. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplatethe iD5 reader’s filter system. Comparing Luminescence sensitivity of SpectraMax Microplate Readers using ATP Assay | Molecular Devices Author: Molecular DevicesMake the most of your budget with the VersaMax™ ELISA Microplate Reader, our affordable, visible spectrum absorbance reader for 96-well microplates. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. 54: 1. No additional manuals are available. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. We would like to show you a description here but the site won’t allow us. 96- or 384-well microplate compatibility. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesa solid, white, flat-bottom 96-well plate. Taking into account the price differences between each seller and each country. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. microplates or in a cuvette at a. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Products : SpectraMax M2/M2e reader, SpectraMax. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. #3610) BrightMax sealing films (Genesee cat. Standard SBS 96 and 384 well plates (128. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. It is a UV/Vis spectrophotometer/microwell reader with built-in cuvette port and microplate drawer. Plate reader instructions Computer. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. Technical specifications. 125 pM. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. of 0. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Figure 2. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. 012 fmol 0. The SpectraMax ABS. No additional manuals are available. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 Japan. 96-well microplate; Immunoassay analyzer;VersaMax price. 2 OD at 200 nm; this decreased to 0. 03 pM 384-well: 0. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. (Figure 1). Shaking: Linear and Orbital. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. #SpectraMax L Config) Methods. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. Optical System: High energy xenon flash lamp. #N207) LAL Reagent Water (Lonza cat. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. The procedure enables direct translation of any published equation. Microplate luminescent reader assay for ERK activity. The Multiskan Sky microplate reader is an absorbance plate reader. 5. Deep well blocks available in 24, 96 and 384-well formats Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. AquaMax 4000 Microplate Washer (Molecular Devices Cat. We would like to show you a description here but the site won’t allow us. Molecular Devices i3x. 98 Bovine serum albumin mg/mL O. Published Dec 16, 2020 | Updated Dec 16, 2020. Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. in a 96-well microplate. We would like to show you a description here but the site won’t allow us. 100 μL/well Steady-Luc working solution was added to the wells. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The FilterMax F3 can read 96-well and 384-well plates. 19 H mm that meet microplate industry standards. Molecular Devices M5 Plate Reader Plate Calibration Form . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. Documentation:1. Wavelength Range. This reader provides visible. Type: Microplate Reader. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Filters: Excitation and emission slides for 4 filters each. It features a modular design that allows users to add or change detection cartridges according to their needs. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . MycoAlert Reagent was added to each well and incubated at room temperature for 5. Compare specifications and request a quote from leading suppliers. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. 5°C at 37°C. flexibility of monochromator-based systems. These models can no longer be repaired. Multimode Reader Comparison Chart. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Measure more accurately. These robust readers have been placed in labs from. analysis software. iD5. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 0. Materials. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . for ValitaTiter Assays. SOP Title: Use and. 6:30 . 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Reader. 1700 Online: Sales Offices. Reading a Microplate . The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Learn more about how absorbance is measured, and some key applications that utilize. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. These robust readers have been placed in labs from Antarctica to. Reconstitution of reagents. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Protein quantitation Cross-talk < 0. Create custom fluorescence assays without having to purchase expensive filter sets. OEM Molecular Devices All Microplate Reader, Top r. Unparalleled performance on a personalized platform. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Minneapolis, MN, USA), according to the manufacturer's protocol. Both readers were heated to 37ºC prior to setting up . Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. High. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. ± 0. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a black, clearbottom microplate. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. CloneMatrix. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Learn more 0200-7008 6- to 1536-well plates. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. A. When reading at wavelengths. spectramax i3x injector cartridge, smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader. " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. /well integration in 384-well luminescence mode Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Read More. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. The eight-channel system delivers both high precision and speed when reading 96-well microplates for endpoint measurements, kinetic assays, and spectral scans. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. 96-well-plate-scan-time-2-colors-1-site-well. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The Perkin Elmer Wallac 1420. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. About;. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. i3x Multi-Mode Microplate Reader. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). The CellTiter-Glo assay from Promega uses luciferase. for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . Brand: MOLECULAR DEVICES. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). microplate readers. < 0. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 2 OD at 200 nm; this decreased to 0. Standard SBS 96 and 384 well plates (128. This can increase throughput and efficiency, thus saving time and space in the lab. Microplate reader assays requiring infrared (IR) or red-shifted. for ValitaTiter Plus standards read on Molecular Devices readers. best-in-class data . #3693; n = 4 for each standard). Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Page: 1/4. DNA concentration (ng/mL) Average RFU. • 0. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. This richly featured reader gives you the speed, convenience, and economy of monochromator-based wavelength selection for visible absorbance measurements between 340 nm and 850. SpectraDrop High-Throughput Screening (HTS) Kit. #M5e) Methods Microplate reader compatibility test. book Page 9 Wednesday, July 7, 2010 2:09 PM. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. #L6876) Guanidine hydrochloride (Sigma cat. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 0 500 1000 1500 2000 2500 3000 3500 time (sec. 63. Cross-talk < 0. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Description. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. 100-μL samples ranging from 1 nM to 10 μM were added to triplicate wells of a solid white 96-well microplate. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Molecular Devices provides. Figure 2-23: Bottle holder To install the bottle holder: 1. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. /well integration in 96-well luminescence mode and 0. Elisa Plate Readers and Microplate Readers at Biocompare. Place the microplate in the drawer. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. It includes both shaking and incubation functions, and is compatible with 96- well microplates. Microplate Capacity Standard 96-well microplates Incubation Temperature control up to 50ºC Temperature uniformity ± 0. Other technologies such as imaging. A multitude of modalities. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 5°C at 37°C. Fluorescence Microplate Readers. 3:40 . 63. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Detergent Compatible Bradford assay. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. In . Never order or replace filters to optimize an assay again!microplate readers from Molecular Devices. Final BSA Concentration (µg/ml) in assay BSA Volume NanoOrange. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. #W50-100) SpectraMax® ABS Plus Microplate Reader. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Can be integrated with Molecular Devices' StakMax® Microplate Stacker. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. Industry standard, 96-well plates are. This page contains the. Comprehensive data analysis software. 3 OD at 200 nm. research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. New SpectraMax iD5 Multi-mode Microplate Reader. #I2511)Introduction. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Supplier: Molecular Devices. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. #ID3-STD) • L-Tryptophan (Sigma cat. Highly customizable low volume applications Molecular Devices unique SpectraDrop Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 0 fmol/well FITC 200 µL in 96 wells. Go to Product Details. field-upgradeable option with one-of-a-kind brightfield cell segmentation, green and red. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 54: 1. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). automation for any lab. ScanLater Western Blot Detection System. Paradigm: Delta mP. 0 OD at. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. 1 mL/well of media. ValitaTiter Plus 96-well (1 plate) VAL004. Table 3 shows sample cell proliferation assay results for CHO-K1 cells plated according to the scheme in Table 1 and allowed to proliferate in culture for two days. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Temperature Control: ambient from +4 to 45C. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. g. Condition: Used - Good. ValitaTiter 96-well (Pack of 20). What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. SpectraMax Mini Multi-Mode Microplate Reader Afordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including. Expt#3 . Detection by Low noise PMT with simultaneous digital and analog modes. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. While they play an important role in generating data from a wide range of. Optimized for fluorescence microplate readers. robotics-automation-compatible. Four wash/rinse bottles are included and configurable for both 96- and 384. The system provides options for 20-, 40-,. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. (a) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. The spectrophotometer has a wide wavelength range 190. 500 USD. Instrument settings can be saved as a protocol file and used repeatedly for reading differentMolecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Efficient cell washing with minimal cell loss and optimal assay performance. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. 3. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. 1 FP • Fluorescence Polarization detection for fluorescein- or . 125 pM * Specifications listed are for europium 96-well: 0. Other Molecular Devices readers with absorbance detection mode, e. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Fluorescence Microplate Reader Comparison Chart. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. for ValitaTiter Plus standards read on Molecular Devices readers. reagent basin for ease of pipetting into. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. 2. Danaher Inc 96 well microplate reader 96 Well Microplate Reader, supplied by Danaher Inc, used in various techniques. Growth medium was then replaced with fatty acid uptake solution. 3% in white 96- and. The reader adds up to three transfers of assay reagents from a 96- or 384. Vmax Kinetic Microplate Reader with SOFTmax® Pro from Molecular Devices, now part of MDS Analytical Technologies. San Jose, California, June 5, 2018. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. 7°C and the bottom to 36. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. *Simple analysis and optimization. Also refer to this Multimode Reader Comparison Chart. (FP) configured microplate readers. Plates can put in the drawer both in landscape and portrait positions. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Microplate readers can be either single-function instruments or “multimode. Temperature Control: Ambient +5C up to 42C. Deep well blocks available in 24, 96 and 384-well formatsFlexStation® 3 Multi-Mode Microplate Readers . To confirm the accurate positioning of arrayed colonies, we performed an absorbance scan in a 96-well scheme with 30 × 30 scans per well for an OmniTray agar plate with 96. Other features and benefits include: Eliminate changing out filters. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. White 96-well plate The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The contents of the SpectraDrop Microplate kit depend on kit type. ***Figure 1. Multimode Readers Comparison Chart. Microplates . The screw remains inside the instrument. The OptiMax by Molecular Devices is a tunable microplate reader. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. Microscopy. #ID3-STD) L-Tryptophan (Sigma cat. We would like to show you a description here but the site won’t allow us. 4 Pages. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Plate Formats: 6 to 384-well microplates. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. Supplementary Information. Specifications for the SpectraMax L microplate reader. We have been using this for DNA measurements 260/280 nm, colorimetric assay measurements like MTT assays, and ELISAs. ©2017 Molecular Devices, LLC . The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. #T-0254) • Lysozyme (Sigma cat. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader.